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Albert Lee

Yeast NDI1 Improve Oxidative Phosphorylation Capacity and Increases Protection Against Oxidative Stress and Cell Death in Cells Carrying a Leber’s Hereditary Optic Neuropathy Mutation

Albert Lee

Mentor: Dr. Yidong Bai

The most common primary mutation found in Leber’s hereditary optic neuropathy is G11778A in the subunit ND4 gene of complex I found in mitochondria. In this study, the NDI1 gene that encodes NADH -quinone oxidoreductase (equivalent to complex I) in the yeast Saccharomyces cerevisiae was introduced into a human cell line, Le1.3.1, which carries the G11778A mutation. The results were two transformant cell lines that showed restored mitochondrial respiration functions, as well as resistance to complex I inhibitor rotenone and restored growth capacity in medium containing galactose. The transformant cells were also relieved of the increased oxidative stress caused by the G11778A mutation, as well as displaying less apoptosis. The results indicate that the NDI1 enzyme of the yeast cells can improve the mitochondrial functions of human cells affected by the G11778A mutation, as well as protect the cells from stress and apoptosis.

Collaborators: Lokendra K. Sharma